What an Endocrine Surgeon Should Know about Sample Collection and Tissue Preservation

Vijay Kumar; Sanjeev Kumar Tripathi; Jai Prakash; Ashish Dubey; Ranjeet Singh Chauhan; Spandana Jagannath; Sabaretnam Mayilvaganan; Sandeep Kumar Singh

doi:10.5005/jp-journals-10088-11214

SEARCH WITHIN CONTENT

Online First

What an Endocrine Surgeon Should Know about Sample Collection and Tissue Preservation

Vijay Kumar, Sanjeev Kumar Tripathi, Jai Prakash, Ashish Dubey, Ranjeet Singh Chauhan, Spandana Jagannath, Sabaretnam Mayilvaganan, Sandeep Kumar Singh

Keywords : Adrenal, Endocrine, Soft tissue tumor, Thyroid cancer

Citation Information : Kumar V, Tripathi SK, Prakash J, Dubey A, Chauhan RS, Jagannath S, Mayilvaganan S, Singh SK. What an Endocrine Surgeon Should Know about Sample Collection and Tissue Preservation. 2023; 18 (2):53-57.

DOI: 10.5005/jp-journals-10088-11214

License: CC BY-NC 4.0

Published Online: 12-01-2024

ARTICLE
REFERENCES

Abstract

One of the challenges that Endocrine Surgeons face is the collection and preservation of tissue samples from patients with endocrine disorders. Different tissue samples are the key essential for the diagnosis and treatment of various endocrine diseases, such as thyroid cancer, adrenal tumors, and parathyroid disorders. However, collecting and preserving tissue samples is a challenge and may hamper the concluding prognosis, onset of the disease, and another parameter of the disease which may mislead the treatment plan of the patients. So, it requires careful planning, preparation, and execution to ensure that the samples are representative of the disease state and suitable for analysis. In this article, we will discuss some of the factors that affect the quality and integrity of tissue samples and provide some tips and best practices for sample collection and preservation.

PDF Share

Misiakos EP, Margari N, Meristoudis C, et al. Cytopathologic diagnosis of fine needle aspiration biopsies of thyroid nodules. World J Clin Cases 2016;4(2):38–48. DOI: 10.12998/wjcc.v4.i2.38.
Berendzen K, Searle I, Ravenscroft D, et al. A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta. Plant Methods 2005;1(1):4. DOI: 10.1186/1746-4811-1-4.
Meng L, Feldman L. A rapid TRIzol-based two-step method for DNA-free RNA extraction from Arabidopsis siliques and dry seeds. Biotechnology Journal 2010;5(2):183–186. DOI: 10.1002/biot.200900211.
Burgess RR, Deutscher MP. Methods in enzymology: Guide to protein purification, 2nd edition. Vol. 436. San Diego: Academic Press; 2009. pp. 16–22.
Ramos-Vara JA, Miller MA. “When tissue antigens and antibodies get along: Revisiting the technical aspects of immunohistochemistry–the red, brown, and blue technique”. Vet Pathol 2014;51(1):42–87. DOI: 10.1177/0300985813505879.

PDF Share